VERIFIKASI METODE DETEKSI PORCINE BERDASARKAN SNI ISO/TS 20224-3: 2020 PADA MATRIKS GELATIN

Auraga Dewantoro, Anna Safarrida, Dianti Rahmasari, Umi Nuraeni, Rika Dwi Susmiarni, Rosalin Damacena, Muhammad Malhan Amin

Abstract

Kebutuhan gelatin untuk memenuhi bahan baku pada produk pangan, farmasi, dan kesehatan terus meningkat. Namun, tingginya impor gelatin yang didominasi membuat kekhawatiran terkait status kehalalan produk gelatin. Salah satu metode deteksi porcine secara molekuler adalah teknik amplifikasi DNA menggunakan qPCR (quantitative polymerase chain reaction). Badan Standardisasi Nasional (BSN) telah mengadopsi salah satu metode potensial terkait deteksi molekuler identifikasi porcine (babi), yaitu SNI ISO/TS 20224-3:2020 dengan lingkup spesies Sus sucrofa dan Sus sucrofa domesticus. Penggunaan metode tersebut belum teridentifikasi untuk penggunaan pada sampel gelatin, sehingga kebutuhan verifikasi metode SNI ISO/TS 20224-3:2020 diperlukan sebagai metode acuan yang terstandar dalam deteksi porcine. Penelitian ini berhasil memverifikasi metode SNI ISO/TS 20224-3:2020 pada sampel gelatin berat 200mg. Kurva amplifikasi real time PCR menggunakan primer spesifik babi menunjukkan bahwa matriks standar gelatin babi teramplifikasi dengan nilai Cq rata-rata 34.56±0.53, dan sampel daging babi teramplifikasi dengan nilai Cq sebesar 18.79±0.28, sedangkan kontrol negatif (daging sapi) tidak menunjukkan kurva amplifikasi. Korelasi data konsentrasi DNA matriks standar gelatin babi, daging babi, dan daging sapi dengan data hasil amplifikasi real time PCR, menunjukkan bahwa semakin besar konsentrasi DNA maka semakin kecil nilai Cq. Sebaliknya, semakin kecil konsentrasi DNA maka semakin besar nilai Cq.Dengan demikian, kandungan DNA porcine semakin tinggi dengan semakin rendahnya nilai Cq.

Keywords

qPCR, halal, Hydrolysis Probe, Cq

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References

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DOI: http://dx.doi.org/10.31153/js.v25i2.993